This Standard Reference Material (SRM®) is intended for use as an accuracy control in the critical evaluation of methods for determining the amount-of-substance concentration of vitamin D metabolites in human serum. This SRM can also be used as a quality assurance tool for assigning values to in-house control materials for these constituents. A unit of NIST-972a consists of four vials (Levels 1 through 4) of frozen serum with different concentration levels of 25-hydroxyvitamin D [25(OH)D] and 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3]. Each vial of NIST-972a contains approximately 1 mL of serum. /// Sample value(s) - please ask for current certificate.
Contents
Analyte
CAS Number
Concentration
Unit
Additional information
Method
25-Hydroxyvitamin D3
[19356-17-3]
28,1 ± 1,1
ng/g
Level 1
25-Hydroxyvitamin D3
[19356-17-3]
28,8 ± 1,1
ng/mL
Level 1
25-Hydroxyvitamin D3
[19356-17-3]
71,8 ± 2,7
nmol/L
Level 1
3-epi-25-hydroxyvitamin D3
[73809-05-9]
1,77 ± 0,10
ng/g
Level 1
3-epi-25-hydroxyvitamin D3
[73809-05-9]
1,81 ± 0,10
ng/mL
Level 1
3-epi-25-hydroxyvitamin D3
[73809-05-9]
4,5 ± 0,2
nmol/L
Level 1
24R,25-Dihydroxyvitamin D3
[40013-87-4]
2,60 ± 0,10
ng/g
Level 1
24R,25-Dihydroxyvitamin D3
[40013-87-4]
2,66 ± 0,10
ng/mL
Level 1
24R,25-Dihydroxyvitamin D3
[40013-87-4]
6,38 ± 0,23
nmol/L
Level 1
25-Hydroxyvitamin D2
[21343-40-8]
0,79 ± 0,08
ng/g
Level 2
25-Hydroxyvitamin D2
[21343-40-8]
0,81 ± 0,06
ng/mL
Level 2
25-Hydroxyvitamin D2
[21343-40-8]
2,0 ± 0,2
nmol/L
Level 2
25-Hydroxyvitamin D3
[19356-17-3]
17,7 ± 0,4
ng/g
Level 2
25-Hydroxyvitamin D3
[19356-17-3]
18,1 ± 0,4
ng/mL
Level 2
25-Hydroxyvitamin D3
[19356-17-3]
45,1 ± 1,0
nmol/L
Level 2
3-epi-25-hydroxyvitamin D3
[73809-05-9]
1,25 ± 0,09
ng/g
Level 2
3-epi-25-hydroxyvitamin D3
[73809-05-9]
1,28 ± 0,09
ng/mL
Level 2
3-epi-25-hydroxyvitamin D3
[73809-05-9]
3,2 ± 0,2
nmol/L
Level 2
24R,25-Dihydroxyvitamin D3
[40013-87-4]
1,38 ± 0,05
ng/g
Level 2
24R,25-Dihydroxyvitamin D3
[40013-87-4]
1,41 ± 0,05
ng/mL
Level 2
24R,25-Dihydroxyvitamin D3
[40013-87-4]
3,39 ± 0,12
nmol/L
Level 2
25-Hydroxyvitamin D2
[21343-40-8]
12,9 ± 0,3
ng/g
Level 3
25-Hydroxyvitamin D2
[21343-40-8]
13,2 ± 0,3
ng/mL
Level 3
25-Hydroxyvitamin D2
[21343-40-8]
32,0 ± 0,8
nmol/L
Level 3
25-Hydroxyvitamin D3
[19356-17-3]
19,4 ± 0,4
ng/g
Level 3
25-Hydroxyvitamin D3
[19356-17-3]
19,8 ± 0,4
ng/mL
Level 3
25-Hydroxyvitamin D3
[19356-17-3]
49,5 ± 1,1
nmol/L
Level 3
24R,25-Dihydroxyvitamin D3
[40013-87-4]
1,58 ± 0,06
ng/g
Level 3
24R,25-Dihydroxyvitamin D3
[40013-87-4]
1,62 ± 0,06
ng/mL
Level 3
24R,25-Dihydroxyvitamin D3
[40013-87-4]
3,88 ± 0,13
nmol/L
Level 3
25-Hydroxyvitamin D3
[19356-17-3]
28,8 ± 0,9
ng/g
Level 4
25-Hydroxyvitamin D3
[19356-17-3]
29,4 ± 0,9
ng/mL
Level 4
25-Hydroxyvitamin D3
[19356-17-3]
73,4 ± 2,3
nmol/L
Level 4
3-epi-25-hydroxyvitamin D3
[73809-05-9]
25,4 ± 2,1
ng/g
Level 4
3-epi-25-hydroxyvitamin D3
[73809-05-9]
26,0 ± 2,2
ng/mL
Level 4
3-epi-25-hydroxyvitamin D3
[73809-05-9]
64,8 ± 5,4
nmol/L
Level 4
24R,25-Dihydroxyvitamin D3
[40013-87-4]
2,58 ± 0,09
ng/g
Level 4
24R,25-Dihydroxyvitamin D3
[40013-87-4]
2,64 ± 0,09
ng/mL
Level 4
24R,25-Dihydroxyvitamin D3
[40013-87-4]
6,32 ± 0,22
nmol/L
Level 4
Miscellaneous
Certificate of Analysis (specimen)
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