This reference material (RM) is intended for harmonizing measurements of abundance and identity using next-generation sequencing-based metagenomics. A unit of RM 8376 consists of 20 tubes (components) containing either bacterial (19 tubes) or human (1 tube) genomic DNA in an aqueous buffer (1 × Tris-EDTA). Each component contains approximately 100 µL of solution. The bacterial components are at a nominal concentration of 50 ng/µL, while the human DNA is nominally 100 ng/µL. /// Sample value(s) - please ask for current report of investigation.
Contents
Analyte
CAS Number
Concentration
Unit
Additional information
Method
Chromosomal Copy Number, Concentration × 10^6
8,84 ± 0,38
copy/µl
A Name: E. coli ATCC®(b) 43895 DNA Organism: Escherichia coli O157:H7
Chromosomal Copy Number, Concentration × 10^7
8,89 ± 0,28
copy/µl
B Name: E. coli ATCC® BAA-2309 DNA Organism: Escherichia coli O104:H4
Chromosomal Copy Number, Concentration × 10^8
9,72 ± 0,38
copy/µl
C Name: S. enterica ATCC® 700720 DNA Organism: Salmonella enterica subsp. enterica
Chromosomal Copy Number, Concentration × 10^9
10,84 ± 0,52
copy/µl
D Name: S. enterica ATCC® 12324 DNA Organism: Salmonella enterica subsp. arizonae
Chromosomal Copy Number, Concentration × 10^10
16,49 ± 0,76
copy/µl
E Name: S. aureus ATCC® BAA-44 DNA Organism: Staphylococcus aureus
Chromosomal Copy Number, Concentration × 10^11
17,38 ± 0,68
copy/µl
F Name: S. aureus ATCC® 12600 DNA Organism: Staphylococcus aureus
Chromosomal Copy Number, Concentration × 10^12
15,99 ± 0,60
copy/µl
G Name: S. epidermidis ATCC® 12228 DNA Organism: Staphylococcus epidermidis
Chromosomal Copy Number, Concentration × 10^13
8,27 ± 0,34
copy/µl
H Name: P. aeruginosa ATCC® BAA-47 DNA Organism: Pseudomonas aeruginosa
Chromosomal Copy Number, Concentration × 10^14
12,01 ± 0,56
copy/µl
I Name: A. baumannii ATCC® 19606 DNA Organism: Acinetobacter baumannii
Chromosomal Copy Number, Concentration × 10^15
21,67 ± 0,94
copy/µl
J Name: N. meningitidis ATCC® 13077 DNA Organism: Neisseria meningitidis
Chromosomal Copy Number, Concentration × 10^16
22,55 ± 0,86
copy/µl
K Name: S. pyogenes ATCC® 12344 DNA Organism: Streptococcus pyogenes
Chromosomal Copy Number, Concentration × 10^17
14,75 ± 0,50
copy/µl
L Name: E. faecalis ATCC® 19433 DNA Organism: Enterococcus faecalis
Chromosomal Copy Number, Concentration × 10^18
7,28 ± 0,36
copy/µl
M Name: A. xylosoxidans ATCC® 27061 DNA Organism: Achromobacter xylosoxidans
Chromosomal Copy Number, Concentration × 10^19
9,97 ± 0,34
copy/µl
N Name: A. hydrophila ATCC® 35654 DNA Organism: Aeromonas hydrophila
Chromosomal Copy Number, Concentration × 10^20
7,68 ± 0,36
copy/µl
O Name: K. pneumoniae ATCC® 13883 DNA Organism: Klebsiella pneumoniae
Chromosomal Copy Number, Concentration × 10^21
9,67 ± 0,36
copy/µl
P Name: S. sonnei ATCC® 25931 DNA Organism: Shigella sonnei
Chromosomal Copy Number, Concentration × 10^22
9,70 ±0,36
copy/µl
Q Name: V. furnissii ATCC® 35016 DNA Organism: Vibrio furnissii
Chromosomal Copy Number, Concentration × 10^23
17,39 ± 0,64
copy/µl
R Name: L. monocytogenes ATCC® 19115 DNA Organism: Listeria monocytogenes
Chromosomal Copy Number, Concentration × 10^24
13,63 ± 0,46
copy/µl
S Name: L. pneumophila ATCC® 33152 DNA Organism: Legionella pneumophila
Chromosomal Copy Number, Concentration × 10^25
0,0323 ± 0,0015
copy/µl
T Name: Homo sapiens GM24385 DNA Organism: Human
Miscellaneous
Certificate of Analysis (specimen)
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Product data sheet
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