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This Standard Reference Material (SRM®) is intended for use as a template for ribonucleic acid (RNA) control synthesis using in vitro transcription (IVT). These RNA controls are designed to be used as external, or ?spike-in?, controls to support confidence in gene expression assays by providing quantitative assessment of the technical performance of a gene expression measurement. A unit of the SRM contains 96 different 0.5 mL polypropylene tubes, with approximately 10 ?g of dehydrated plasmid deoxyribonucleic acid (DNA) in each. Each tube contains plasmid DNA with a unique template sequence for a different external RNA control. These controls were developed in cooperation with the External RNA Control Consortium (ERCC).
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NIST provides over 1300 Standard Reference Materials® as well as articles and practice guides describing the development, analysis and use of SRMs.
NIST Standard Reference Materials are used by analytical laboratories in industry, academia and government in order to facilitate commerce and trade and to advance research and development. The SRMs are produced by the engineering laboratory, the material measurement laboratory, and the physical measurement laboratory at NIST. They are available for chemical composition, physical properties, and engineering materials.