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Description

The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).Spectral PropertiesAbsorbance: 662nmEmission: 694nm