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Description

BioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.Spectral PropertiesAbsorbance: 408nmEmission: 452nm