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Description

Glutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals. Alterations in glutathione homeostasis are associated with a variety of diseases, therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes.The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.Spectral PropertiesAbsorbance: 520nm Emission: 625nm and 582nm