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Description

Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome. Because Simplicon is a synthetic RNA, there is no risk of the transgenes(s) integrating into the transfected cell's genome. Moreover, the self-replicating nature of the Simplicon means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc. The Simplicon TagRFP RNA kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagRFP RNA kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagRFP expresses a monomeric red (orange) fluorescent protein generated from the RFP of sea anemone Entacmaea quadricolor. TagRFP exhibits fluorescence withexcitation/emission maxima at 555/584 nm respectively, and brightness that is nearly three times higher than mCherry.