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Description

X-Gal is a chromogenic substrate used in conjunction with IPTG for blue/white colony selection. It is a soluble colorless compound comprising galactose attached to a substituted indole. In the presence of X-Gal the lac+ ,bacteria after ?-complementation form blue colonies and bacteria carrying recombinant plasmid form white colonies.

Structure formula

5-Bromo-4-chloro-3-indolyl beta-D-Galactopyranoside

Contents

Miscellaneous

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