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Description

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST.Tag, His.Tag, and S.Tag coding sequences, and a C-terminal HSV.Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase. pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation. For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.