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Description

pBAC-3 is a baculovirus transfer plasmid compatible with BacVector-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His.Tag and/or S.Tag sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.