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Description

Application Note: 3,6-Diacetoxy-fluorescein Bis(methylene)bis(azanetriyl)tetraacetic Acid is an intermediate used in the synthesis of Calcein AM (C125400), which is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.

Structure formula

3,6-Diacetoxy Fluorescein Bis(methylene)bis(azanetriyl)tetraacetic Acid

Contents

Miscellaneous

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TRC | Toronto Research Chemicals

Quality analytical standards and reference materials for food and environmental analysis, research

Founded in 1982, Toronto Research Chemicals is specialized in the development and production of reference materials used in research as well as food safety testing and environmental analysis. The range of more than 350,000 products includes reference standards for pesticides and metabolites, impurities, natural compounds and complex organic small molecules not otherwise commercially available.