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Description

"BioTracker MitoBlue Live Cell Dye is a synthetic bisbenzamidine derivative that selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching in both live and fixed cells (1). It is a unique mitochondrial dye in that after initially staining mitochondria, the dye is transported to lysosomes in a time-dependent manner. This distinct staining pattern allows for monitoring and tracking the mitochondrial recycling mechanism in living cells (2). MitoBlue has been shown to be suitable for two-photon microscopy using a 740 nm two-photon excitation (TPE) wavelength and emission channel at 480 nm (2).Spectral PropertiesAbsorbance: 329nmEmission: 420nmReference:1. Sa?nchez, Mateo I., et al. ""MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria."" ACS chemical biology 9.12 (2014): 2742-2747.2. Sánchez, Mateo I., et al. ""MitoBlue as a tool to analyze the mitochondria-lysosome communication."" Scientific reports 10.1 (2020): 1-12."