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Description

SimpliconMBOL> is a novel system to effect immediate high sustained protein expression of multiple genes into transfected cells without the risk of genome integration. The technology employs a single, synthetic, polycistronic, self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1,2,3. The SimpliconMBOL> RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been removed and replaced with the transgenes of interest.

Introduction and replication of the SimpliconMBOL> RNA is expected to elicit a strong interferon response in transfected cells. To suppress the IFN responses, a Vaccinia virus protein4, B18R, is used for the original SimpliconTM technology.

The B18R RNA (human codon optimized) is a synthetic mRNA that is co-transfected with the SimpliconMBOL> RNA to suppress the IFN responses. B18R RNA can also be used to prepare B18R conditioned medium by transfection into HFFs (Cat. No. SCC058). B18R RNA can also be used for co-transfection of any mRNAs to suppress the IFN responses and enable repeated transfection of mRNAs. Please refer to the User Guide for the SimpliconMBOL> Expression System located on our website for detailed protocols.