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Description

Endothelial Cell Characterization Kit Antibodies for the immunocytochemical characterization of endothelial cells., Endothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon's endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques. Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry: * Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL * Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL * Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions: * Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL * Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml