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Description

Prokineticins, also known as endocrine gland vascular endothelial growth factors (EG-VEGF), are two ~10 kD secreted proteins originally described to mediate angiogenesis and gastrointestinal smooth muscle contraction (Li et al., 2001, LeCouter et al., 2003). Subsequently, prokineticins have been found to mediate central nervous system functions including circadian rhythms and olfactory bulb development (Cheng et al., 2002, Ng et al., 2005). Two Gq-coupled receptors, PK1 and PK2 (also known as GPR73a and GPR73b), mediate cellular responses to prokineticins (Lin et al., 2002). Millipore's PK2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression, thus, they are ideal HTS tools for screening of agonists and antagonists at PK2. The membrane preparations exhibit a Kd of 0.36 nM for [125I]-MIT-1. With 0.3 nM [125I]-MIT-1, 10mug/well PK2 Membrane Prep typically yields greater than 5-fold signal-to-background ratio., Full-length human GPR73L1 cDNA encoding PK2