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Description

Galanin is a 29-30 amino acid peptide originally purified from intestine but later found to be abundant in the CNS. A family of 3 GPCRs, GAL1, GAL2 and GAL3, bind to galanin and mediate its biological effects. Galanin and its receptors have been implicated in pain, cognition, seizure activity, depression and drug addiction (Hokfelt, 2005). In particular, GAL1 mediates the anticonvulsant activity of galanin, mice lacking GAL1 are affected by spontaneous seizures, and have increased susceptibility to seizures induced by lithium-pilocarpine treatment and electrical perforant path stimulation (McColl et al., 2006, Mazarati et al., 2004). Chemicon's GAL1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression, thus, they are ideal HTS tools for screening of antagonists of GAL1 interactions with galanin. The membrane preparations exhibit a Kd of 1.7 nM for [125I]-galanin. With 5 ug/well GAL1 Membrane Prep and 0.25 nM [125I]-galanin, a greater than 50-fold signal-to-background ratio was obtained., Human GAL1