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Description

Millipore's Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. Neuromedin U (NmU) is a peptide characterized by its ability to promote smooth muscle contraction (Brighton et al., 2004a). Two GPCRs, NMU1 and NMU2, mediate the contractile effects of neuromedin U by activation of both Gq and Gi (Brighton et al., 2004b). In addition, NmU binds to NMU1 expressed on Th2 cells to induce cytokine release (Johnson et al., 2004). Millipore's cloned human NMU1 expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NMU1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at NMU1.