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Description

Millipore's Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response. P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. A wide variety of cells and tissues express P2Y1. Vascular endothelium and smooth muscle express P2Y1, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation (Ralevic and Burnstock, 1998). Millipore's cloned human P2Y1-expressing cell line is made in the 1321N1 host, which supports high levels of recombinant P2Y1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at P2Y1.