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Description

Corning Matrigel Matrix for Organoid Culture is a solubilized basement membrane preparation that has been optimized for organoid culture. Verified to support both mouse and human organoid growth and differentiation from healthy and/or diseased tissue, each lot has a specific elastic modulus value indicating the stiffness of the gel formed and is qualified to form stable 3D domes commonly used in organoid culture protocols. Available in 10mL LDEV-free, Phenol Red-free format.Corning Matrigel Matrix for Organoid culture is extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.Quality. ,Tested for ability to form and maintain stable 3D domes/droplets on multi-well plates.. , Elastic Modulus (matrix stiffness) measured using a rheometer. . , Protein concentrations determined by Lowry method. , Mouse colonies routinely screened for pathogens via Mouse antibody Production (MAP) testing. ,Extensive PCR testing is performed to screen for a number of pathogens. ,Tested and found negative for bacteria, fungi, and mycoplasma. ,Endotoxin units measured by Limulus Amoebocyte Lysate assay. ,Matrigel Matrix gel stability is tested for a period of 14 days at 37C