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Description

Corning Fibronectin, human, 5mg, is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.QualityPurity = 90% by SDS-PAGETested for ability to promote attachment and spreading of BHK-21 cellsFound negative for bacteria, fungi, and mycoplasmaSourceHuman plasma. Source material tested for hepatitis B antigen and HIV-1 antibody.Molecular Weight440 kD (unreduced form)Preparation and StorageStable for three months at 2 to 8C (lyophilized) or two weeks at -20C (solubilized)HandlingRecommended concentration - 1-5 mug/cm² of growth surface for coating or at 5 mug/ml as a media additiveFormulationLyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0