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Description

Corning Laminin, mouse, 1mg, is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.QualityPurity >90% by SDS-PAGE (contains entactin)Tested for ability to initiate neurite outgrowth of NG-108 human neuroblastoma cellsFound negative for bacteria, fungi, and mycoplasmaSourceEngelbreth-Holm-Swarm (EHS) mouse tumorFormulationFrozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4Molecular Weight900 kD (unreduced)Preparation and StorageStable for at least three months at -70C.HandlingRecommended concentration: 1-10 mug/cm² of growth surface depending on cell type