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Description

PromoCell Cell Pellets are stored in RNAlater(R) - an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Note: RNAlater(R) denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater(R) Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 µ,l RNAlater(R). The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.