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Description

Insect POPCULTURE(R) Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His&bull,Bind affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.