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"I use GeneJuice because it's ease-of-use, low toxicity and wide range of target cells""""Stuart Rulten. Research Fellow. University of Sussex""""Since we have been working with GeneJuice, we have saved a lot of time and money!""""Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany""""I find that Genejuice works for a multitude of cell types with minimal optimization""""Caitriona Marie Lyons. PhD Student. University College Cork, IrelandTransfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates., Cell lines transfected with GeneJuice(R) Transfection ReagentCell Lines:Primary Cells:10T1/2293T3T3 NIH3T3 Swiss3T3-L1A204A431A549alpha TC1-6AR 42JAs4.1AtT-20B50BC-1BC-2BC3BCBLBHK-21C3H/10T1/2C6C2C12Caco-2Caki-1Calpan-1Calu-1Calu-6CCL-131CFPAC-1Chang LiverCHOCHO-7CHO-IRCHO-K1COS-1COS-7CS-1CV-1DaudiDDTI MF-2DT40ECV304EL4ES-E14TG2aEVSCC17MH9c2HCT-116HEK293HeLaHeLa BHeLa T4Hep 3B2.1-7HepG2Hepa 1-6Ht-29HTB-37HTB-45Huh-7HUVECIC21IEC-6JEG-3JurkatKBL57-3-11L-6L-929MA-10McA-RH7777MCF-7MCF-10-2AMDCKMelanocyteMG-63Neuro 2ANeuroblastomaNRKNT2/D1OV-1063OVCAR3P4P19PC12PA317PAM212PS-1R2CRAW 264.7RBL-2H3RMP-41SAOS-2SC-1Schneider line2SK-N-MCSK-N-SHSKOV3STOSW-480SW-837T3M4TM4U937UCDVero Aortic smooth muscle cells Astrocytes Angioblasts Chondrocytes Chromaffin cells Epithelial cells: mammary prostate tracheal Fibroblasts Keratinocytes, Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells."