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Description

Streptavidin Agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination. Provided as a 50% slurry in storage buffer, 5 ml settled resin in 10 ml total volume., Column or batch isolation of biotinylated molecules, SpecificationsConcentration50% suspension (5 ml settled volume)Storage buffer100 mM sodium phosphate pH 7.5, 0.02% sodium azideSize40-165 µ,mBinding capacity>85 nmol free biotin/mlProteaseNone detected (SDS-PAGE of purified protein)DNaseNone detected (blue/white colony assay of ligated plasmid)RNaseNone detected (gel assay of purified RNA)