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Description

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore., Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke&prime,s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Structure formula

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin

Contents

Miscellaneous

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