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Description

An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-alpha Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (kcat = 21.6 s-1 when the substrate concentration is half the Vmax, S0.5 = 342 µ,M). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm, Emission max: ~420 nm., An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µ,M). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.