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Description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm, emission max.: ~490), based on the GVNLDAEF sequence derived from the beta-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by beta-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µ,M) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37C)., A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the beta-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by beta-secretase results in fluorescence enhancement. kcat/Km values (min-1/µ,M) at 37C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.