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Description

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Absorbance: <=0.2 at 300 nm, and <=0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer., A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: <=0.2 at 300 nm, and <=0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.