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Description

A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µ,M), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µ,M. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.