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Description

Many membrane receptors for neurotransmitters and hormones are coupled to intracellular effectors and ion channels by heterotrimeric G-proteins. Receptor activation by ligand causes exchange, in a catalytic fashion, of GDP by GTP on the beta-subunits of heterotrimeric G-proteins, followed by the dissociation of the beta-subunit from the betagamma-subunits. The latter reaction leads to regulation of a variety of effector systems. Recent evidence suggests that in addition to the beta-subunit the betagamma-subunits may also play a role in effector system regulation. The betagamma-subunit has been implicated in the regulation of K+ channels, phospholipase C and A2, and type II adenylylcyclase. A recent study demonstrated a role for betagamma-subunits in targeting the beta-adrenergic receptor kinase to membrane-bound receptors as well. This product is useful for quantification of beta-subunit present in any particular cellular compartment and to study the role of G-protein in intracellular signaling. The purified standard is suitable for positive control or can be used to construct a standard curve using Anti-G-Protein, beta-Subunit, Internal (127-139) RAbbit pAb (Cat. No. 371738)., Native Gbetagamma-subunit purified from bovine brain. Provides sufficient G-protein betagamma-subunit for six standard curves. The betagamma-subunit is biologically active and can be used in reconstitution experiments. Useful for quantitation of beta-subunit present in any particular cellular compartment.