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Description

Orange Serum Agar is used for the isolation, cultivation and bacterial count of acid-tolerant microorganisms that cause deterioration of fruit juices and fruit-juice concentrates, particularly those of citrus fruits. It is generally used in fruit-juice production and to confirm the presence of acid-tolerant microorganisms in food packaging materials. Casein peptone serves the growth requirements such as carbon and nitrogen compounds, yeast extract provides B-complex vitamins and trace elements. Glucose is also a source of carbon and energy. In combination with the low pH value of the medium, the additional acid formation from glucose favors the growth of yeasts, molds, Lactobacillus sp. and Leuconostoc sp. These spoilage agents are particularly stimulated by the addition of orange extract (orange serum).