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Description

ReadyPlate(TM) Tryptone Bile X-Glucuronide Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of &beta,-glucuronidase positive Escherichia coli from food and feeding stuff. The presence of the enzyme &beta,-D-glucuronidase differentiates most Escherichia coli from other coliforms. E. coli absorbs the chromogenic substrate 5-bromo-4-chloro-3-indolyl-&beta,-D glucuronide (X-&beta,-D-glucuronide). The enzyme &beta,-glucuronidase splits the bond between the chromophore 5-bromo-4-chloro-3-indolyle- and the &beta,-D-glucuronide forming blue to blue-green colored colonies. The growth of accompanying Gram-positive flora is largely inhibited by the use of bile salts and the high incubation temperature of 44C.