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Description

MacConkey Agar is a weakly selective medium for the isolation of Enterobacteriaceae or specifically E. coli. Enterobacteriaceae can be clearly identified even in the presence of Salmonella and Shigella. The medium inhibits the growth of Gram-positive bacteria due to the presence of crystal violet and bile salts, whereas it supports the growth of most Gram-negative bacteria. Bacteria degrading lactose to acids grow in pink to red colored colonies. Additionally, E. coli and other acid forming bacteria will show a zone of precipitated bile salt around the colonies. Bacteria not degrading lactose will grow colorless. The plates are single bagged in transparent, hydrogen peroxide impermeable sleeves (non-irradiated). The sleeves consist of polypropylene with a barrier of PE-EVOH-PE.