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Description

mTSB (modified Tryptic Soy Broth) without the addition of novobiocin is a selective enrichment media for the isolation and growth of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) and Salmonella from meat products, carcass, and environmental sponges. For the selective enrichment of Escherichia coli O157:H7 in outbreak-related samples, e.g. fermented sausage, cooked meat products, the addition of novobiocin is recommended. Pancreatic digest of casein, papaic digest of soybean meal, and casamino acids (casein acid hydrolysate) serve as carbon and nitrogen sources, vitamins, and minerals. Glucose also serves as a carbon energy source for rapid growth during the enrichment. Sodium chloride maintains the osmotic balance and dipotassium hydrogen phosphate acts as a buffering agent for maintaining the pH balance of the medium. The addition of Bile salts ensures selectivity of the media.