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Description

Linden Grain Medium (Base) is a granulated dehydrated culture media with high nutrient content. It is used for microbiological validation of aseptic filling lines across the beverage industry because it creates optimum growth conditions for fungi and bacteria at various pH levels. The pH of the medium can be adjusted to the filling line criteria. At a pH of 4.3, fungi and bacteria which are adapted to grow under high acid conditions show visible growth within 7 days of incubation at 28C. Bacteria that grow best at neutral to slightly alkaline pH, like Bacillus subtilis, show no growth/very limited growth at pH 4.3. Any growth within the medium (turbidity), on the surface of the medium, or the inner wall or bottom of the tubes and bottles indicates the presence of spoilage organisms. Experiments have shown that the medium could also be used after freezing and thawing, thus making this medium suitable for ice crusher installations.