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Description

Tumor necrosis factor alpha (TNF-alpha) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-alpha is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-alpha is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn's disease, psoriasis and refractory asthma. TNF-alpha also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication., The SMC(R) Mouse TNF-alpha Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC(R) immunoassay system detects analytes using Single Molecule Counting (SMC(R)) technology.