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Beschreibung

Mitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is lost when mitochondria become depolarized during cell death, allowing monitoring of cell viability. The BioTracker 633 Red Mitochondria Dye can be detected using the red Cy5 channel. The fluorescence is dependent on mitochondrial membrane potential, and the dye can be used to monitor changes in mitochondrial membrane potential in cells during apoptosis.Spectral PropertiesAbsorbance: 622nmEmission: 648nm