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Beschreibung

CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.Materials Provided: One vial containing 2 µ,g (40 µ,L) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/muL.Validation: Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µ,L (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.CpGenome is a trademark of Serologicals Corporation.