Zurück zu Ihrem Suchergebnis

Beschreibung

Human herpesvirus-8 (also known as Kaposi's sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. The most prominent protein expressed in latently infected cells is the ORF73 gene product known as the latency-associated nuclear antigen (LANA) that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. The ORF59 (also known as PF-8), a phosphoprotein, is reported to be a processivity factor for HHV-8 DNA polymerase (Pol-8). It interacts with and stimulates Pol-8 DNA synthesis activity. It is an early-late gene product localized to HHV-8-infected cell nuclei. PF-8 interacts with HHV-8 DNA polymerase (Pol-8) and stimulates Pol-8 DNA synthesis activity. Amino acid residues 10-27 and 279-301 are reported to be critical for the processivity function of PF-8. Residues 10 to 27 are essential for its interaction with Pol-8 and 279-301 are required for its binding to dsDNA. Clone 11D1 specifically detects PF-8 of HHV-8 and exhibits predominant nuclear membrane fluorescence in TPA (phorbol ester)-induced, HHV-infected BCBL-1 cells. (Ref.: Strahan, RC., et al. (2017). PLoS Pathog 13(7), e1006482, Chan, SR., and Chandran, B (2000). J. Virol. 74(23), 10920-10929, Chan, SR., et al. (1998). 240: 118-126).