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Category: Fluorescent Labels and Indicators, MTS and Sulfhydryl Active Reagents /// Appearance: Yellow Solid /// Application Notes: A water soluble bifunctional fluorogenic reagent for the sensitive and specific detection of thiols in biological specimens or proteins.SBD-F has high reactivity to thiol compounds to attain low detection limits, no flourescence in itself, biological buffer solublility, stability at RT in water for >1 week. The optimal condition for reaction was 60oC and pH 9.5 for 1 h.Please refer to the following references for more specific information regarding the application of SBD-F as a fluorogenic reagent for detection of cysteine containing peptides.Fluorescence: max Abs. l = 380 nm, max Em. l = 515 nm, Molar Abs. > 4x103 (ca. 317nm) /// References: Imai, K., et al.: Anal. Biochem., 128, 471 (1983)| Sueyoshi, T., et al.: J. Biochem., 97, 1811 (1985), Lagana, A., et al.: Anal. Biochem., 215, 266 (1993)| Singh, B.K., et al.: J. Liq. Chromatogr., 17, 4469 (1994)| Bishop, A.P., et al.: Free Radical Biol. Med., 18, 617 (1995)|