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Beschreibung

DNA01 is Plasmid pGem7zf+ (Promega) which is linearized with the SmaI endonuclease. A linear DNA molecules (3000 b. p.) are deposited onto freshly cleaved mica. Molecules are uniformly distributed on the surface with molecule density - 0,5-7 molec./um2 and typical DNA length 1009nm. Recommended humidity for obtaining a good image is 3-5%.