Zurück zu Ihrem Suchergebnis

Beschreibung

Spike glycoprotein (UniProt: K9N5Q8, also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 3) gene (Gene ID: 14254594) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Spike protein is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form that can be cleaved into three subunits known as S1 (aa 18-751), S2 (aa 752-1353), and S2 (aa 888-163) by host cell furin or other cellular proteases. Spike protein has an extracellular domain (aa 18-1296), a transmembrane domain (aa 1297-1317), and a cytoplasmic domain (aa 1318-1353). S1 subunit attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. It interacts with host DPP4 to mediate viral entry. The S2 subunit mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Clone m336 binds to the receptor binding domain of spike protein that is localized in amino acids 360-601. It is shown to neutralized both pseudotyped and live MERS-CoV with high potency likely by competing with DPP4 for binding to the S glycoprotein. It does not exhibit reactivity with SARS-CoV2 spike protein. (Ref.: Ying, T., et al. (2014). J. Virol. 88(14), 7796-7805).