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Beschreibung

Introduction: Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. A family of cell surface receptors, the integrins, mediates cell attachment to proteins of the extracellular matrix. Integrins are heterodimer molecules containing an alpha and beta transmembrane glycoprotein subunit that are non-covalently bound together (1-2). Historically, antibodies have been used to determine the integrin profiles of cells by immunoprecipitation, immunofluorescence, immunoblotting, and flow cytometry. These methods are time-consuming, laborious or require sophisticated equipment. The CHEMICON(R) Beta Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of beta integrin surface expression. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: . Alpha Integrin-Mediated Cell Adhesion Array (ECM530) . Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (ECM532) . QCM 8mum 96-well Chemotaxis Cell Migration Assay (ECM510) . QCM 5mum 96-well Chemotaxis Cell Migration Assay (ECM512) . QCM 3mum 96-well Chemotaxis Cell Migration Assay (ECM515) . QCM 96-well Cell Invasion Assay (ECM555) . 24-well Insert Cell Migration and Invasion Assay Systems . CytoMatrix Cell Adhesion strips (ECM protein coated) . QuantiMatrix ECM protein ELISA kits Test Principle: The CHEMICON Beta Integrin-Mediated Cell Adhesion Array uses mouse monoclonal antibodies generated against human beta integrins/subunits (Ã,eta1, Ã,eta2, Ã,eta3, Ã,eta4, Ã,eta6, alphaVÃ,eta5, and alpha5Ã,eta1), that are immobilized onto a goat anti-mouse antibody coated microtiter plate. The plate is then used to capture cells expressing these integrins on their cell surface, goat anti-mouse antibody coated wells are provided as a negative assay control. Each strip (8 wells total) consists of 7 integrin antibody coated wells and one negative control well (see plate layout on page 3). Experimental cells are seeded onto the coated substrate and incubated. Subsequently, unbound cells are washed away, and adherent cells are fixed and stained. Relative cell attachment is determined using absorbance readings. Additional information about the monoclonals provided in the kit: *Mouse anti-Beta 1 [CD29], clone P4G11, (Hu, Mky, Rb) *Mouse anti-Beta 2 [CD18], clone P4H9, (Hu) *Mouse anti-Beta 3 [CD61], clone 25E11, (Hu) *Mouse anti-Beta 4 [CD104], clone ASC-9, (Hu) *Mouse anti-Beta 6, clone CSb6, (Hu, Rt, Mky, Rb) *Mouse anti-Alpha V/ Beta 5 [CD51/CD61], clone P1F6, (Hu, Chk) *Mouse anti-Alpha 5/ Beta 1 [VLA5, FNR], clone HA5, (Hu, Por)