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Beschreibung

NovaTaq DNA polymerase is a recombinant form of Thermus aquaticus DNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaq DNA polymerase exhibits 5?-3? DNA polymerase activity and lacks 3?- 5? exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3?-dA overhangs, suitable for cloning with Novagen(R)Perfectly Blunt(R), AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli Concentration: 5.0 U/?l Purity: 95% homogeneous by SDS-PAGEEndonuclease: None detected Amplification effiency: Functional PCR Storage: -20C