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Beschreibung

Absorbance (1.0 M, H2O, 260 nm):<=0.05.Absorbance (1.0 M, H2O, 280 nm): <=0.04, A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37C). Exhibits no metal binding and has a small temperature dependent DeltapKa of -0.014/C., A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25C.