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Beschreibung

A cell permeable hydrozide that activates AMPK indirectly via a CaMKKbeta-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKalpha2 subunit. This activator promotes lysosomal amyloid-beta degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µ,M. Treatment with RSVA314 at 1-3 µ,M results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots., A cell permeable hydrozide that activates AMPK indirectly via a CaMKKbeta-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKalpha2 subunit. This activator promotes lysosomal amyloid-beta degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µ,M. Treatment with RSVA314 at 1-3 µ,M results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.